Effects of methylene blue and curcumin photosensitizers on the color stability of endodontically treated intraradicular dentin.

BACKGROUND: Photodynamic therapy with photosensitizers can reduce the microbial load. However, few studies have evaluated the effects of photosensitizers on the color stability of endodontically treated intraradicular dentin. This in vitro study investigated the effects of methylene blue and curcumin photosensitizers used in photodynamic therapy on the color stability of intraradicular dentin. METHODS: Sixty human incisors were divided into three experimental groups according to the photosensitizer solutions used and their concentrations: methylene blue (25 mg/L), curcumin (1000 mg/L), and curcumin (1500 mg/L). The color stability of endodontically treated intraradicular dentin was evaluated using a portable reflectance spectrophotometer before and after the samples been storage in 2 mL of the photosensitizer solutions during 5 min (n = 20). Color stability data were subjected to a normality test, and statistical analysis was performed using the one-way analysis of variance and Tukey least significant difference test (α = 0.05). RESULTS: Samples treated with 25 mg/L methylene blue photosensitizer showed a higher level of color alteration than those stored in 1000 mg/L curcumin (p = .03322). However, there was no significant difference in the color alteration profiles between the samples treated with 25 mg/L methylene blue and 1500 mg/L curcumin (p = .36428). Furthermore, there was no difference in the color alteration profiles between the dentin samples immersed in 1000 mg/L and 1500 mg/L curcumin photosensitizer solutions (p = .45321). CONCLUSIONS: Methylene blue and curcumin photosensitizers influenced the color stability of endodontically treated intraradicular dentin, and this color alteration exceeded the clinical acceptability threshold. Samples treated with 25 mg/L methylene blue showed the highest level of color alteration.

Influência da potência de luz de refletores odontológicos na polimerização precoce de compósitos / Dental light potency influence on the precocious polymerization of composites

O objetivo deste trabalho é avaliar a influência de diferentes potências dos refletores de luzaplicadas no campo operatório e sua relação com a polimerização precoce de resinas compostas em diversas opacidades, empregando-se iluminadores com tecnologia LED e com luz halógena. Para a confecção dos corpos de prova foram utilizadas as resinas compostas nanohíbrida Esthet X HD (Dentsply, Caulk - Milford, DE). nas tonalidades de cor A20; A2 e YE apresentados em corpos de prova 5mm de diâmetro e 1 mm de espessura. Os corpos de prova foram então expostos aos refletores LED (Gnatus Equipamentos Médico-Odontológicos Ltda/SP - Brasil) e Halógeno (Gnatus Equipamentos Médico-Odontológicos Ltda/SP - Brasil) durante 2 minutos, para cada tipo de lâmpada (LED e Halógena) e nas potências de luz (15.000 e 35.000 lux). A análise do grau de conversão dos monômeros em polímeros foi obtida com o uso de um espectrofotômetro (FTIR640, Varian) por meio de espectroscopia de infravermelho. A Análise de Variância (Anova Two Way) revelou diferenças estatisticamente significantes para as duas potências de luz avaliadas (p<0.001). Comparações pareadas foram realizadas através da aplicação dos testes Tukey, revelando diferenças nas potências testadas para cada opacidade de resina. Conclui-se baseado nos resultados encontrados que existe uma relação de dependência na densidade da potenciade luz empregada nos refletores de iluminação da cavidade bucal durante os procedimentos clínicos que se reflete no grau de conversão de compostos fotoativados.

The goal of this study was to evaluate the influence of different light potencies irradiated on the dentistry operative field and its relation with the precocious polymerization of composite resin in various opacities, employing LED and Halogen Dentallights. Specimens were prepared using nanohíbrid composites HD Esthet X (Dentsply Caulk - Milford, DE), in shades A20, A2 and YE submitted in samples 1 mm thick and 5 mm in diameter then exposed to LEDs (Gnatus Medical Dental Equipment Ltd I SP - Brazil) and Halogen Dental Light (Gnatus Medical Dental Equipment Ltd I SP - Brazil) irradiated for 2 min, for each type of lamp (LED and Halogen) and power light of 15.000 and 35.000 lux. The conversion degree of monomers into polymers was obtained by means of infrared spectroscopy (FTIR). Analysis ofVariance (ANOVA-Two way) revealed significant differences for the different powers of tested light (p <0.001). Pairwise comparisons were performed using the Tukey tests, revealing statistically significant differences for the different tested light potencies for each resin opacity. So, based on obtained results it can be stated that there is a dependency relation of light potency density used in illuminationof thé oral cavity during the clinical procedures which is reflected in the degree conversion of photo activated compounds.